LOCUS       P75957                   233 aa            linear   BCT 25-JAN-2005
DEFINITION  Lipoprotein releasing system ATP-binding protein lolD.
ACCESSION   P75957
VERSION     P75957  GI:3915974
DBSOURCE    swissprot: locus LOLD_ECOLI, accession P75957;
            class: standard.
            extra accessions:Q9R7N6,created: Nov 1, 1997.
            sequence updated: Dec 15, 1998.
            annotation updated: Jan 25, 2005.
            xrefs: gi: 48994873, gi: 1787361, gi: 1651548, gi: 4062689
            xrefs (non-sequence databases): HSSPQ58206, EchoBASEEB3214,
            EcoGeneEG13440, InterProIPR003439, PfamPF00005, ProDomPD000006,
            PROSITEPS00211, PROSITEPS50893
KEYWORDS    ATP-binding; Complete proteome; Direct protein sequencing; Inner
            membrane; Membrane; Transport.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 233)
  AUTHORS   Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1474 (1997)
   PUBMED   9278503
  REMARK    NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
            STRAIN=K12 / MG1655
REFERENCE   2  (residues 1 to 233)
  AUTHORS   Oshima,T., Aiba,H., Baba,T., Fujita,K., Hayashi,K., Honjo,A.,
            Ikemoto,K., Inada,T., Itoh,T., Kajihara,M., Kanai,K., Kashimoto,K.,
            Kimura,S., Kitagawa,M., Makino,K., Masuda,S., Miki,T.,
            Mizobuchi,K., Mori,H., Motomura,K., Nakamura,Y., Nashimoto,H.,
            Nishio,Y., Saito,N., Sampei,G., Seki,Y., Tagami,H., Takemoto,K.,
            Wada,C., Yamamoto,Y., Yano,M. and Horiuchi,T.
  TITLE     A 718-kb DNA sequence of the Escherichia coli K-12 genome
            corresponding to the 12.7-28.0 min region on the linkage map
  JOURNAL   DNA Res. 3 (3), 137-155 (1996)
   PUBMED   8905232
  REMARK    NUCLEOTIDE SEQUENCE.
            STRAIN=K12
REFERENCE   3  (residues 1 to 233)
  AUTHORS   Yakushi,T., Masuda,K., Narita,S., Matsuyama,S. and Tokuda,H.
  TITLE     A new ABC transporter mediating the detachment of lipid-modified
            proteins from membranes
  JOURNAL   Nat. Cell Biol. 2 (4), 212-218 (2000)
   PUBMED   10783239
  REMARK    PROTEIN SEQUENCE OF 1-11, MUTAGENESIS OF GLY-42, AND
            CHARACTERIZATION.
REFERENCE   4  (residues 1 to 233)
  AUTHORS   Narita,S., Tanaka,K., Matsuyama,S. and Tokuda,H.
  TITLE     Disruption of lolCDE, encoding an ATP-binding cassette transporter,
            is lethal for Escherichia coli and prevents release of lipoproteins
            from the inner membrane
  JOURNAL   J. Bacteriol. 184 (5), 1417-1422 (2002)
   PUBMED   11844772
  REMARK    CHARACTERIZATION, AND SUBUNIT.
COMMENT     On Nov 23, 1998 this sequence version replaced gi:2829637.
            [FUNCTION] Part of the ABC transporter complex lolCDE involved in
            the translocation of mature outer membrane-directed lipoproteins,
            from the inner membrane to the periplasmic chaperone, lolA.
            Responsible for the formation of the lolA-lipoprotein complex in an
            ATP-dependent manner. Such a release is dependent of the
            sorting-signal (absence of an Asp at position 2 of the mature
            lipoprotein) and of lolA.
            [SUBUNIT] The complex is composed of two ATP-binding proteins
            (lolD) and two transmembrane proteins (lolC and lolE).
            [SUBCELLULAR LOCATION] Inner membrane-associated.
            [SIMILARITY] Belongs to the ABC transporter family. Lipoprotein
            translocase (TC 3.A.1.125) subfamily.
            -------------------------------------------------------------------
            -------This Swiss-Prot entry is copyright. It is produced through a
            collaboration between  the Swiss Institute of Bioinformatics  and
            the  EMBL outstation -the European Bioinformatics Institute.  There
            are no  restrictions on  its use as long as its content is in no
            way modified and this statement is not removed.
            -------------------------------------------------------------------
            -------.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..233
                     /gene="lolD"
                     /locus_tag="b1117"
     Protein         1..233
                     /gene="lolD"
                     /locus_tag="b1117"
                     /product="Lipoprotein releasing system ATP-binding protein
                     lolD"
     Site            42..49
                     /gene="lolD"
                     /locus_tag="b1117"
                     /site_type="np-binding"
                     /note="ATP (By similarity)."
                     /evidence=experimental
     Site            42
                     /gene="lolD"
                     /locus_tag="b1117"
                     /site_type="mutagenized"
                     /note="G->D: Loss of lipoprotein release when
                     overexpressed."
                     /evidence=experimental
ORIGIN      
        1 mnkillqcdn lckryqegsv qtdvlhnvsf svgegemmai vgssgsgkst llhllggldt
       61 ptsgdvifng qpmsklssaa kaelrnqklg fiyqfhhllp dftalenvam plligkkkpa
      121 einsraleml kavgldhran hrpselsgge rqrvaiaral vnnprlvlad eptgnldarn
      181 adsifqllge lnrlqgtafl vvthdlqlak rmsrqlemrd grltaelslm gae
//